Plasmid
Part:BBa_K3394008:Design
Designed by: Talia Shaler Group: iGEM20_BGU-Israel (2020-10-17)
Cellulolytic complex [2] with AIDA-I
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal EcoRI site found at 2117
Illegal EcoRI site found at 2813
Illegal EcoRI site found at 6015
Illegal PstI site found at 2165
Illegal PstI site found at 2751
Illegal PstI site found at 5263 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal EcoRI site found at 2117
Illegal EcoRI site found at 2813
Illegal EcoRI site found at 6015
Illegal PstI site found at 2165
Illegal PstI site found at 2751
Illegal PstI site found at 5263 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal EcoRI site found at 2117
Illegal EcoRI site found at 2813
Illegal EcoRI site found at 6015
Illegal BamHI site found at 28
Illegal BamHI site found at 3015 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal EcoRI site found at 2117
Illegal EcoRI site found at 2813
Illegal EcoRI site found at 6015
Illegal PstI site found at 2165
Illegal PstI site found at 2751
Illegal PstI site found at 5263 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal EcoRI site found at 2117
Illegal EcoRI site found at 2813
Illegal EcoRI site found at 6015
Illegal PstI site found at 2165
Illegal PstI site found at 2751
Illegal PstI site found at 5263
Illegal NgoMIV site found at 3227
Illegal NgoMIV site found at 3274
Illegal NgoMIV site found at 3642
Illegal NgoMIV site found at 3802
Illegal NgoMIV site found at 4156
Illegal AgeI site found at 51 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5089
Design Notes
We designed the order of genes in the system.
Source
Artificial sequence - Synthetic construct
References
Jose, J., & Meyer, T. F. (2007). The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications. Microbiology and Molecular Biology Reviews, 71(4), 600–619.
Amir, L., Carnally, S. A., Rayo, J., Rosenne, S., Melamed Yerushalmi, S., Schlesinger, O., ... & Alfonta, L. (2013). Surface display of a redox enzyme and its site-specific wiring to gold electrodes. Journal of the American Chemical Society, 135(1), 70-73.